The roles of two clip domain serine proteases in innate immune responses of the malaria vector Anopheles gambiae
- Author(s)
- Volz, J; Osta, MA; Kafatos, FC; Muller, HM;
- Details
- Publication Year 2005-12-02,Volume 280,Issue #48,Page 40161-40168
- Journal Title
- JOURNAL OF BIOLOGICAL CHEMISTRY
- Publication Type
- Journal Article
- Abstract
- The malaria vector Anopheles gambiae is capable of multiple immune responses against Plasmodium ookinetes. Accumulating evidence in several insect species suggests the involvement of serine protease cascades in the initiation and coordination of immune responses. We report molecular and reverse genetic characterization of two mosquito clip domain serine proteases, CLIPB14 and CLIPB15, which share structural similarity to proteases involved in prophenoloxidase activation in other insects. Both CLIPs are expressed in mosquito hemocytes and are transcriptionally induced by bacterial and Plasmodium challenges. Functional studies applying RNA interference revealed that both CLIPs are involved in the killing of Plasmodium ookinetes in Anopheles. Studies on parasite melanization demonstrated an additional role for CLIPB14 in the prophenoloxidase cascade. We further report that both CLIPs participate in defense toward Gram-negative bacteria. Our findings strongly suggest that clip domain serine proteases serve multiple functions and play distinctive roles in several immune pathways of A. gambiae.
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Keywords
- PRO-PHENOL-OXIDASE; PREPHENOLOXIDASE-ACTIVATING ENZYME; HOLOTRICHIA-DIOMPHALIA LARVAE; QUANTITATIVE TRAIT LOCI; COMPLEMENT-LIKE PROTEIN; MANDUCA-SEXTA SERPIN-4; BOMBYX-MORI; HORSESHOE-CRAB; CDNA CLONING; PROPHENOLOXIDASE
- Publisher's Version
- https://doi.org/10.1074/jbc.M506191200
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- Refer to copyright notice on published article.
Creation Date: 2005-12-02 12:00:00