Unexpectedly, induction of cytotoxic T lymphocytes enhances the humoral response after DNA immunization
- Details
- Publication Year 2004-04-15,Volume 103,Issue #8,Page 3073-3075
- Journal Title
- BLOOD
- Publication Type
- Journal Article
- Abstract
- Although there are many examples (eg, immune deviation) in which enhanced cellular responses correspond with lower humoral responses, here we demonstrate for the first time 2 models in which cytotoxic T-lymphocyte (CTL) activity is associated with an enhanced antibody response. First, C57BL/6 mice generate a stronger antibody response to ovalbumin DNA immunization than congenic bm1 mice. The latter differ from C57BL/6 mice in that the H-2K(b) molecule is mutated so that the immunodominant CTL epitope of ovalbumin is no longer presented. Second, pre-existing CTLs (induced by ovalbumin peptide-priming) increased the antibody response to a second unrelated antigen (P-galactosidase) co-immunized with ovalbumin. One possible mechanism is that CTLs may release antigen from DNA-transfected cells by killing or damaging them, and this freed antigen is then accessible to dendritic cells and B cells. Our finding of CTL-mediated antibody enhancement has important implications for tumor and viral immunoblology and vaccination.
- Publisher
- AMER SOC HEMATOLOGY
- Keywords
- DENDRITIC CELLS; IN-VIVO; IMMUNITY; PEPTIDE; IDENTIFICATION; ANTIBODY; ANTIGEN
- Publisher's Version
- https://doi.org/10.1182/blood-2003-07-2305
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2004-04-15 12:00:00