Binding site for the C-domain of insulin-like growth factor (IGF) binding protein-6 on IGF-II; implications for inhibition of IGF actions

Author(s): Headey, SJ; Keizer, DW; Yao, SG; Wallace, JC; Bach, LA; Norton, RS;
Details: Publication Year 2004-06-18,Volume 568,Issue #1-3,Page 19-22
Journal Title: FEBS LETTERS
Publication Type: Journal Article
Abstract: Insulin-like growth factors (IGFs) are important mediators of growth and IGF-binding proteins (IGFBPs) 1-6 regulate IGF actions. As lGFBP C-terminal domains contribute to high-affinity IGF binding, we have defined the binding site for the C-domain of IGFBP-6 on IGF-II using NMR. This site lies adjacent to and between the binding sites for the IGFBP N-domain and IGF-I receptor (IGFIR), which have previously been found on opposite sides of the IGF molecule. The C-domain is therefore likely to interfere with IGF binding to the IGFIR, providing a structural basis for the potent inhibitory effects of intact IGFBPs on IGF actions. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publisher: ELSEVIER SCIENCE BV
Keywords: DISEASE ANTIGEN OSPA; TERMINAL DOMAIN; RECEPTOR; EXPRESSION; MUTANTS; IDENTIFICATION; EPITOPES; DELETION; IGFBP-5; REGION
Publisher's Version: https://doi.org/10.1016/j.febslet.2004.04.091
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2004-06-18 12:00:00