SOCS5 is expressed in primary B and T lymphoid cells but is dispensable for lymphocyte production and function

Brender, C; Columbus, R; Metcalf, D; Handman, E; Starr, R; Huntington, N; Tarlinton, D; Odum, N; Nicholson, SE; Nicola, NA; Hilton, DJ; Alexander, WS

Author(s): Brender, C; Columbus, R; Metcalf, D; Handman, E; Starr, R; Huntington, N; Tarlinton, D; Odum, N; Nicholson, SE; Nicola, NA; Hilton, DJ; Alexander, WS;
Details: Publication Year 2004-07,Volume 24,Issue #13,Page 6094-6103
Journal Title: MOLECULAR AND CELLULAR BIOLOGY
Publication Type: Journal Article
Abstract: Suppressors of cytokine signaling (SOCSs) are key regulators of cytokine-induced responses in hematopoietic as well as nonhematopoietic cells. SOCS1 and SOCS3 have been shown to modulate T-cell responses, whereas the roles of other SOCS family members in the regulation of lymphocyte function are less clear. Here, we report the generation of mice with a targeted disruption of the Socs5 gene. Socs5(-/-) mice were born in a normal Mendelian ratio and were healthy and fertile. We found that SOCS5 is expressed in primary B and T cells in wild-type mice. However, no abnormalities in the lymphocyte compartment were seen in SOCS5-deficient mice. We examined antigen- and cytokine-induced proliferative responses in B and T cells in the absence of SOCS5 and found no deviations from the responses seen in wild-type cells. Because SOCS5 has been implicated in Th1 differentiation, we also investigated the importance of SOCS5 in T helper cell responses. Unexpectedly, SOCS5-deficient CD4 T cells showed no abnormalities in Th1/Th2 differentiation and Socs5(-/-) mice showed normal resistance to infection with Leishmania major. Therefore, although SOCS5 is expressed in primary B and T cells, it appears to be dispensable for the regulation of lymphocyte function.
Publisher: AMER SOC MICROBIOLOGY
Keywords: MICE LACKING SUPPRESSOR; INDUCED MACROPHAGE ACTIVATION; CYTOKINE SIGNALING SOCS; JANUS TYROSINE KINASE; LEISHMANIA-MAJOR; NEGATIVE REGULATION; CUTTING EDGE; BOX MOTIF; IN-VIVO; C-MPL
Publisher's Version: https://doi.org/10.1128/MCB.24.13.6094-6103.2004
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Creation Date: 2004-07-01 12:00:00