Differential production of inflammatory chemokines by murine dendritic cell subsets

Proietto, AI; O&#39;Keeffe, M; Gartlan, K; Wright, MD; Shortman, K; Wu, L; Lahoud, MH

Author(s): Proietto, AI; O'Keeffe, M; Gartlan, K; Wright, MD; Shortman, K; Wu, L; Lahoud, MH;
Details: Publication Year 2004,Volume 209,Issue #1-2,Page 163-172
Journal Title: IMMUNOBIOLOGY
Publication Type: Journal Article
Abstract: Dendritic cells (DC) are efficient antigen presenting cells with the ability to activate naive T cells. Murine DC represent a heterogeneous population that can be subdivided into distinct subsets, including the conventional DC (cDC) which are either CD4(-)CD8(-) (DN), CD4(+)CD8(-) (CD4(+)) or CD4(-)CD8(+) (CD8(+)) subsets and the plasmacytoid DC (pDC), which have different immune regulatory functions. In this study, we investigated the differential expression of genes encoding the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes, and the secretion of these chemokines, among splenic DC subsets. These chemokine genes were expressed at higher levels by the splenic CD4(+) and DN cDC subsets compared with the CD8(+) cDC, in both the resting and activated states in vivo. Both the pDC and cDC subsets displayed increases in chemokine secretion in response to a range of toll-like receptor (TLR) stimuli in vitro. Whilst the pDC were the highest producers of Mip-1alpha and Mip-1beta in response to some TLR stimuli, the DN and CD4(+) cDC subsets were the superior producers of Rantes. Overall, of the cDC, the CD4(+) cDC produced all chemokines most efficiently, both at a basal level, and in response to most TLR stimuli. Thus, we report a new functional difference between the murine splenic cDC subsets, with the CD4(+) cDC demonstrating the most efficient production of the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes. (C) 2004 Elsevier GmbH. All rights reserved.
Publisher: URBAN & FISCHER VERLAG
Keywords: MOUSE LYMPH-NODES; RECEPTOR EXPRESSION; CUTTING EDGE; IN-VIVO; T-CELLS; SURFACE PHENOTYPE; CD8 EXPRESSION; HUMAN BLOOD; SPLEEN; SUBTYPES
Publisher's Version: https://doi.org/10.1016/j.imbio.2004.03.002
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Creation Date: 2004-01-01 12:00:00