SOCS3 is a critical physiological negative regulator of G-CSF signaling and emergency granulopoiesis
Details
Publication Year 2004-02, Volume 20, Issue #2, Page 153-165
Journal Title
IMMUNITY
Publication Type
Journal Article
Abstract
To determine the importance of suppressor of cytokine signaling-3 (SOCS3) in the regulation of hematopoietic growth factor signaling generally, and of G-CSFinduced cellular responses specifically, we created mice in which the Socs3 gene was deleted in all hematopoietic cells. Although normal until young adulthood, these mice then developed neutrophilia and a spectrum of inflammatory pathologies. When stimulated with G-CSF in vitro, SOCS3-deficient cells of the neutrophilic granulocyte lineage exhibited prolonged STAT3 activation and enhanced cellular responses to G-CSF, including an increase in cloning frequency, survival, and proliferative capacity. Consistent with the in vitro findings, mutant mice injected with G-CSF displayed enhanced neutrophilia, progenitor cell mobilization, and splenomegaly, but unexpectedly also developed inflammatory neutrophil infiltration into multiple tissues and consequent hind-leg paresis. We conclude that SOCS3 is a key negative regulator of G-CSF signaling in myeloid cells and that this is of particular significance during G-CSF-driven emergency granulopoiesis.
Publisher
CELL PRESS
Keywords
COLONY-STIMULATING FACTOR; BLOOD PROGENITOR CELLS; CYTOKINE SIGNALING-3; PERIPHERAL-BLOOD; FACTOR-RECEPTOR; IN-VIVO; HUMAN-NEUTROPHILS; REPORTER STRAIN; TRANSGENIC MICE; BONE-MARROW
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2004-02-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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