Autointmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1(+) myeloid progenitor cells that differentiate in vivo into resting dendritic cells
- Author(s)
- Steptoe, RJ; Ritchie, JM; Jones, LK; Harrison, LC;
- Details
- Publication Year 2005-02-01,Volume 54,Issue #2,Page 434-442
- Journal Title
- DIABETES
- Publication Type
- Journal Article
- Abstract
- The nature of the T-cell response to antigen is governed by the activation state of the antigen-presenting dendritic cell (DC). Immature or resting DCs have been shown to induce T-cell responses that may protect against the development of autoimmune disease. Effectively harnessing this "tolerogenic" effect of resting DCs requires that it be disease-specific and that activation of DCs by manipulation ex vivo is avoided. We reasoned that this could be achieved by transferring in vivo partially differentiated myeloid progenitor cells encoding a disease-specific autoantigen. With the aim of preventing antoimmune diabetes, we transferred myeloid progenitor cells encoding proinsulin into NOD mice. Bone marrow (BM) was cultured in granulocyte macrophage colony-stimulating factor (GM-CSF) and transforming growth factor-beta1, a cytokine combination that expands myeloid cells but inhibits terminal DC differentiation, to yield Gr-1(+)/CD11b(+)/CD11c(-) myeloid progenitor cells and a minor population of CD11c(+)/ CD11b(+)/CD86(1o) immature DCs. After transfer, Gr-l(+) myeloid cells acquired the characteristics of resting DCs (CD11c(+)/MHC classII(int)/CD86(lo)/CD40(lo)). Gr-1(+) myeloid cells generated from transgenic NOD mice that expressed proinsulin controlled by a major histocompatibility complex (MHC) class II promoter, but not from wild-type NOD mice, transferred into 4-week-old female NOD mice significantly suppressed diabetes development. The transfer of DC progenitors encoding a disease-specific autoantigen is, therefore, an effective immunotherapeutic strategy that could be applied to humans.
- Publisher
- AMER DIABETES ASSOC
- Keywords
- MOUSE BONE-MARROW; NOD MICE; T-CELLS; ANTIGEN; TOLERANCE; EXPRESSION; MONOCYTES; PRECURSORS; UNRESPONSIVENESS; COSTIMULATION
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Creation Date: 2005-02-01 12:00:00