Early appearance of germinal center-derived memory B cells and plasma cells in blood after primary immunization
- Author(s)
- Blink, EJ; Light, A; Kallies, A; Nutt, SL; Hodgkin, PD; Tarlinton, DM;
- Details
- Publication Year 2005-02-21,Volume 201,Issue #4,Page 545-554
- Journal Title
- JOURNAL OF EXPERIMENTAL MEDICINE
- Publication Type
- Journal Article
- Abstract
- Immunization with a T cell-dependent antigen elicits production of specific memory B cells and antibody-secreting cells (ASCs). The kinetic and developmental relationships between these populations and the phenotypic forms they and their precursors may take remain unclear. Therefore, we examined the early stages of a primary immune response, focusing on the appearance of antigen-specific B cells in blood. Within 1 wk, antigen-specific B cells appear in the blood with either a memory phenotype or as immunoglobulin (Ig)G1 ASCs expressing blimp-1. The memory cells have mutated V-H genes; respond to the chemokine CXCL13 but not CXCL12, suggesting recirculation to secondary lymphoid organs; uniformly express 13220; show limited differentiation potential unless stimulated by antigen; and develop independently of blimp-1 expression. The antigen-specific IgG1 ASCs in blood show affinity maturation paralleling that of bone marrow ASCs, raising the possibility that this compartment is established directly by blood-borne ASCs. We find no evidence for a blimp-1-expressing preplasma memory compartment, suggesting germinal center output is restricted to ASCs and B220(+) memory B cells, and this is sufficient to account for the process of affinity maturation.
- Publisher
- ROCKEFELLER UNIV PRESS
- Keywords
- ANTIBODY-FORMING-CELLS; PRIMARY IMMUNE-RESPONSE; BONE-MARROW; AFFINITY MATURATION; IMMUNOLOGICAL MEMORY; CLONAL SELECTION; SECRETING CELLS; EXPRESSION; ANTIGEN; MICE
- Publisher's Version
- https://doi.org/10.1084/jem.20042060
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2005-02-21 12:00:00