Early appearance of germinal center-derived memory B cells and plasma cells in blood after primary immunization
Details
Publication Year 2005-02-21, Volume 201, Issue #4, Page 545-554
Journal Title
JOURNAL OF EXPERIMENTAL MEDICINE
Publication Type
Journal Article
Abstract
Immunization with a T cell-dependent antigen elicits production of specific memory B cells and antibody-secreting cells (ASCs). The kinetic and developmental relationships between these populations and the phenotypic forms they and their precursors may take remain unclear. Therefore, we examined the early stages of a primary immune response, focusing on the appearance of antigen-specific B cells in blood. Within 1 wk, antigen-specific B cells appear in the blood with either a memory phenotype or as immunoglobulin (Ig)G1 ASCs expressing blimp-1. The memory cells have mutated V-H genes; respond to the chemokine CXCL13 but not CXCL12, suggesting recirculation to secondary lymphoid organs; uniformly express 13220; show limited differentiation potential unless stimulated by antigen; and develop independently of blimp-1 expression. The antigen-specific IgG1 ASCs in blood show affinity maturation paralleling that of bone marrow ASCs, raising the possibility that this compartment is established directly by blood-borne ASCs. We find no evidence for a blimp-1-expressing preplasma memory compartment, suggesting germinal center output is restricted to ASCs and B220(+) memory B cells, and this is sufficient to account for the process of affinity maturation.
Publisher
ROCKEFELLER UNIV PRESS
Keywords
ANTIBODY-FORMING-CELLS; PRIMARY IMMUNE-RESPONSE; BONE-MARROW; AFFINITY MATURATION; IMMUNOLOGICAL MEMORY; CLONAL SELECTION; SECRETING CELLS; EXPRESSION; ANTIGEN; MICE
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Creation Date: 2005-02-21 12:00:00
Last Modified: 0001-01-01 12:00:00
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