In vitro and in vivo assays for osteoclast apoptosis

Author(s): Akiyama, T; Miyazaki, T; Bouillet, P; Nakamura, K; Strasser, A; Tanaka, S;
Journal Title: BIOLOGICAL PROCEDURES ONLINE
Publication Type: Journal Article
Abstract: Mature osteoclasts, multinucleated giant cells responsible for bone resorption, are terminally differentiated cells with a short life span. Recently, we have demonstrated that osteoclast apoptosis is regulated by ERK activity and Bcl-2 family member Bim. In this paper, we summarize the methods we used to study osteoclast apoptosis in vitro and in vivo. Using adenovirus and retrovirus vectors, we were able to introduce foreign genes into osteoclasts and examine their effects on osteoclast survival in vitro. In addition, we established the modified methods for in situ hybridization and BrdU labeling of bone sections from mice to study osteoclast survival in vivo. The detailed methods described here could be useful for studying the biological process in bone.
Publisher: BIOMED CENTRAL LTD
Keywords: BCL-2 FAMILY-MEMBER; BH3-ONLY PROTEIN BIM; NF-KAPPA-B; C-SRC; SURVIVAL; ADENOVIRUS; ACTIVATION; COMPLEX; KINASE; CELLS
Publisher's Version: https://doi.org/10.1251/bpo105
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2005-05-09 12:00:00