Plasma cell ontogeny defined by quantitative changes in Blimp-1 expression
Publication Year 2004-10-18, Volume 200, Issue #8, Page 967-977
- Journal Title
- JOURNAL OF EXPERIMENTAL MEDICINE
- Publication Type
- Journal Article
- Plasma cells comprise a population of terminally differentiated B cells that are dependent on the transcriptional regulator B lymphocyte induced maturation protein 1 (Blimp-1) for their development. We have introduced a gfp reporter into the Blimp-1 locus and shown that heterozygous mice express the green fluorescent protein in all antibody-secreting cells (ASCs) in vivo and in vitro. In vitro, these cells display considerable heterogeneity in surface phenotype, immunoglobulin secretion rate, and Blimp-1 expression levels. Importantly, analysis of in vivo ASCs induced by immunization reveals a developmental pathway in which increasing levels of Blimp-1 expression define developmental stages of plasma cell differentiation that have many phenotypic and molecular correlates. Thus, maturation from transient plasmablast to long-lived ASCs in bone marrow is predicated on quantitative increases in Blimp-1 expression.
- ROCKEFELLER UNIV PRESS
- TRANSCRIPTION FACTOR XBP-1; PRIMARY IMMUNE-RESPONSE; GENE-EXPRESSION; B-LYMPHOCYTES; C-MYC; SECRETING CELLS; MATURE B; DIFFERENTIATION; REPRESSION; MATURATION
- Publisher's Version
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Creation Date: 2004-10-18 12:00:00Last Modified: 0001-01-01 12:00:00