LIF receptor signaling modulates neural stem cell renewal
Details
Publication Year 2004-11, Volume 27, Issue #3, Page 255-266
Journal Title
MOLECULAR AND CELLULAR NEUROSCIENCE
Publication Type
Journal Article
Abstract
Activation of the leukemia inhibitory factor (LIF) receptor has been reported to promote gliogenesis and also to support neural stem cell (NSC) renewal. To investigate this paradox, we isolated NSCs and generated neurospheres from embryonic mice either wild-type, heterozygous, or homozygous null for LIF receptor (LIFR)-beta. Exogenous LIF abrogated neurosphere formation and promoted expression of GFAP by all cells in wild-type and heterozygous cultures. LIF also stimulated a twofold increase in the number of multipotential clones generated from these cultures in comparison with those pretreated with EGF and FGF-2 (E+F) alone. In contrast, the clonogenicity of low-density cultures of LIFR knockout cells was reduced in comparison with that of wild-type cells grown in E+F and was unaffected by LIF. Thus, although LIFR signaling is not necessary for NSC self-renewal, it enhances both the clonogenicity and the expression of GFAP by these multipotential cells. (C) 2004 Elsevier Inc. All rights reserved.
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Keywords
LEUKEMIA INHIBITORY FACTOR; CILIARY NEUROTROPHIC FACTOR; CENTRAL-NERVOUS-SYSTEM; ADULT-MOUSE BRAIN; MAMMALIAN FOREBRAIN; NEURONAL DIFFERENTIATION; GENERATION; MAINTENANCE; ASTROCYTES; CYTOKINES
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2004-11-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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