Murine megakaryocyte progenitor cells and their susceptibility to suppression by G-CSF
Details
Publication Year 2005-01, Volume 23, Issue #1, Page 55-62
Journal Title
STEM CELLS
Publication Type
Journal Article
Abstract
In agar cultures of mouse bone marrow cells, megakaryocyte colony-forming cells exhibited shorter survival times than granulocyte-macrophage progenitor cells when initially cultured in the absence of stimulating factors. Initiation of cultures with G-CSF improved the survival times of granulocyte-macrophage progenitor cells and those of megakaryocyte progenitor cells. Paradoxically, G-CSF was found to consistently inhibit megakaryocyte colony formation stimulated by erythropoietin or by stem cell factor plus interleukin-3 (IL-3) plus erythropoietin. G-CSF was a less-consistent inhibitor of megakaryocyte colonies stimulated by thrombopoietin or IL-3. Analysis of the response of marrow cells from mice with the deletion of the genes encoding CIS, SOCS-1, SOCS-2, SOCS-3, SOCS-5, SOCS-6, or SOCS-7 indicated that the inhibitory SOCS proteins, with the possible exception of SOCS-3, were not involved in the G-CSF-initiated suppression of megakaryocyte colony formation.
Publisher
ALPHAMED PRESS
Keywords
COLONY-STIMULATING FACTOR; MOUSE HEMATOPOIETIC-CELLS; MICE LACKING SUPPRESSOR; IN-VITRO; STEM-CELLS; C-MPL; THROMBOPOIETIN; LIGAND; GROWTH; GRANULOPOIESIS
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2005-01-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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