Expression of Aire and the early wave of apoptosis in spermatogenesis
- Author(s)
- Schaller, CE; Wang, CL; Beck-Engeser, G; Goss, L; Scott, HS; Anderson, MS; Wabl, M;
- Details
- Publication Year 2008-02-01,Volume 180,Issue #3,Page 1338-1343
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Expression of the autoimmune regulator (Aire) protein in mice and humans is thought to be restricted to the medullary epithelial and monocyte-dendritic cells of the thymus. There it mediates expression and presentation of a large variety of proteins, including those that are peripheral organ-specific and are not expressed by other thymocytes. In this way, self-reactive T lymphocytes that would attack peripheral cells producing these proteins are confronted with the self-Ags and, as a consequence, are deleted. In this study, we show that Aire mRNA is also expressed in the testis-another tissue with promiscuous gene expression. Aire protein, however, is expressed only sporadically in spermatogonia and spermatocytes. Transcription of genes that are under Aire control in the thymus is unaffected by Aire in the testis. However, in mice with a disrupted Aire gene, the scheduled apoptotic wave of germ cells, which is necessary for normal mature spermatogenesis, is reduced, and sporadic apoptosis in adults is increased. Because Rag-1 deficiency does not abolish the effect, the adaptive immune system is not involved. We suggest that there is a link between the scheduled and sporadic apoptotic processes and propose that scheduled apoptosis provides a counterselection mechanism that keeps the germline stable.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- AUTOIMMUNE REGULATOR AIRE; GERM-CELL APOPTOSIS; DEFICIENT MICE; GENE; PROTEIN; MOUSE; DNMT3L; THYMUS; PMS2; TOLERANCE
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- Refer to copyright notice on published article.
Creation Date: 2008-02-01 12:00:00