Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice
- Author(s)
- Robertson, EJ; Charatsi, I; Joyner, CJ; Koonce, CH; Morgan, M; Islam, A; Paterson, C; Lejsek, E; Arnold, SJ; Kallies, A; Nutt, SL; Bikoff, EK;
- Details
- Publication Year 2007-12-15,Volume 134,Issue #24,Page 4335-4345
- Journal Title
- DEVELOPMENT
- Publication Type
- Journal Article
- Abstract
- The zinc-finger transcriptional repressor Blimp1 (Prdm1) controls gene expression patterns during differentiation of B lymphocytes and regulates epigenetic changes required for specification of primordial germ cells. Blimp1 is dynamically expressed at diverse tissue sites in the developing mouse embryo, but its functional role remains unknown because Blimp1 mutant embryos arrest at E10.5 due to placental insufficiency. To explore Blimp1 activities at later stages in the embryo proper, here we used a conditional inactivation strategy. A Blimp1-Cre transgenic strain was also exploited to generate a fate map of Blimp1-expressing cells. Blimp1 plays essential roles in multipotent progenitor cell populations in the posterior forelimb, caudal pharyngeal arches, secondary heart field and sensory vibrissae and maintains key signalling centres at these diverse tissues sites. Interestingly, embryos carrying a hypomorphic Blimp1(gfp) reporter allele survive to late gestation and exhibit similar, but less severe developmental abnormalities, whereas transheterozygous Blimp1(gfp/-) embryos with further reduced expression levels, display exacerbated defects. Collectively, the present experiments demonstrate that Blimp1 requirements in diverse cell types are exquisitely dose dependent.
- Publisher
- COMPANY OF BIOLOGISTS LTD
- Keywords
- TRANSCRIPTIONAL REPRESSOR BLIMP-1; MATURATION PROTEIN-1 BLIMP-1; PLASMA-CELL DIFFERENTIATION; OUTFLOW TRACT DEVELOPMENT; PRIMORDIAL GERM-CELLS; EARLY MOUSE EMBRYO; NEURAL CREST; GENE-EXPRESSION; VERTEBRATE LIMB; TERMINAL DIFFERENTIATION
- Publisher's Version
- https://doi.org/10.1242/dev.012047
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2007-12-15 12:00:00