Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo
Details
Publication Year 2007-11, Volume 8, Issue #11, Page 1217-1226
Journal Title
NATURE IMMUNOLOGY
Publication Type
Journal Article
Abstract
The development of functionally specialized subtypes of dendritic cells ( DCs) can be modeled through the culture of bone marrow with the ligand for the cytokine receptor Flt3. Such cultures produce DCs resembling spleen plasmacytoid DCs ( pDCs), CD8(+) conventional DCs (cDCs) and CD8(-) cDCs. Here we isolated two sequential DC-committed precursor cells from such cultures: dividing 'pro-DCs', which gave rise to transitional 'pre-DCs' en route to differentiating into the three distinct DC subtypes (pDCs, CD8(+) cDCs and CD8(-) cDCs). We also isolated an in vivo equivalent of the DC-committed pro-DC precursor cell, which also gave rise to the three DC subtypes. Clonal analysis of the progeny of individual pro-DC precursors demonstrated that some pro-DC precursors gave rise to all three DC subtypes, some produced cDCs but not pDCs, and some were fully committed to a single DC subtype. Thus, commitment to particular DC subtypes begins mainly at this pro-DC stage.
Publisher
NATURE PUBLISHING GROUP
Keywords
BONE-MARROW CULTURES; COLONY-STIMULATING FACTOR; FLT3 LIGAND; HEMATOPOIETIC PRECURSORS; STEADY-STATE; PROGENITORS; MONOCYTES; DISTINCT; SPLEEN; MICE
Publisher's Version
https://doi.org/10.1038/ni1522
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2007-11-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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