Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo
- Author(s)
- Naik, SH; Sathe, P; Park, HY; Metcalf, D; Proietto, AI; Dakic, A; Carotta, S; O'Keeffe, M; Bahlo, M; Papenfuss, A; Kwak, JY; Wu, L; Shortman, K;
- Details
- Publication Year 2007-11,Volume 8,Issue #11,Page 1217-1226
- Journal Title
- NATURE IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- The development of functionally specialized subtypes of dendritic cells ( DCs) can be modeled through the culture of bone marrow with the ligand for the cytokine receptor Flt3. Such cultures produce DCs resembling spleen plasmacytoid DCs ( pDCs), CD8(+) conventional DCs (cDCs) and CD8(-) cDCs. Here we isolated two sequential DC-committed precursor cells from such cultures: dividing 'pro-DCs', which gave rise to transitional 'pre-DCs' en route to differentiating into the three distinct DC subtypes (pDCs, CD8(+) cDCs and CD8(-) cDCs). We also isolated an in vivo equivalent of the DC-committed pro-DC precursor cell, which also gave rise to the three DC subtypes. Clonal analysis of the progeny of individual pro-DC precursors demonstrated that some pro-DC precursors gave rise to all three DC subtypes, some produced cDCs but not pDCs, and some were fully committed to a single DC subtype. Thus, commitment to particular DC subtypes begins mainly at this pro-DC stage.
- Publisher
- NATURE PUBLISHING GROUP
- Keywords
- BONE-MARROW CULTURES; COLONY-STIMULATING FACTOR; FLT3 LIGAND; HEMATOPOIETIC PRECURSORS; STEADY-STATE; PROGENITORS; MONOCYTES; DISTINCT; SPLEEN; MICE
- Publisher's Version
- https://doi.org/10.1038/ni1522
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2007-11-01 12:00:00