Clonogenic mast cell progenitors and their excess numbers in chimeric BALB/c mice with inactivated GATA-1
Details
Publication Year 2007-11-20, Volume 104, Issue #47, Page 18642-18647
Journal Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type
Journal Article
Abstract
In agar cultures of marrow cells from adult female BALB/c chimeric GATA-1(Plt13/+) mice, a high frequency of unusual dispersed colonies was noted. Analysis showed that these were colonies of mast cells and that mast cell colony-forming cells (progenitors) could be detected in clonal cultures of adult marrow, neonatal marrow, or fetal liver if the combined stimulus of stem cell factor and interleukin-3 was used. Mast cell progenitors were in active cell cycle and showed an extensive capacity for self-generation. Mast cell colonies both from control GATA-1(+/+) mice and GATA-1(Plt13/+) mice could generate growth factor-dependent cloned cell lines that grew for > 18 months. Surprisingly, the majority of the excessive numbers of mast cell progenitors in chimeric GATA-1(Plt13/+) mice were transcribing the inactive Plt13 allele of GATA-1, suggesting that GATA-11 normally acts to restrict the emergence of committed mast cell progenitors. In sharp contrast, all eosinophil progenitors in these mice were transcribing the normal GATA-1 allele. No excess tissue mast cells were observed in GATA-(1Plt13/+) mice, suggesting that the excess mast cell progenitors in these mice might be generating mast cells with a defective in vivo proliferative or tissue homing capacity.
Publisher
NATL ACAD SCIENCES
Keywords
IN-VIVO; MOUSE; DIFFERENTIATION; THROMBOPOIETIN; MATURATION; MUTATION; LACKING; INVIVO; ADULT; ROLES
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2007-11-20 12:00:00
Last Modified: 0001-01-01 12:00:00
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