Autoantigen-specific interactions with CD4(+) thymocytes control mature medullary thymic epithelial cell cellularity
Details
Publication Year 2008-09-19,Volume 29,Issue #3,Page 451-463
Journal Title
IMMUNITY
Publication Type
Journal Article
Abstract
Medullary thymic epithelial cells (mTECs) are specialized for inducing central immunological tolerance to self-antigens. To accomplish this, mTECs must adopt a mature phenotype characterized by expression of the autoimmune regulator Aire, which activates the transcription of numerous genes encoding tissue-restricted self-antigens. The mechanisms that control mature Aire(+) mTEC development in the postnatal thymus remain poorly understood. We demonstrate here that, although either CD4(+) or CD8(+) thymocytes are sufficient to sustain formation of a well-defined medulla, expansion of the mature mTEC population requires autoantigen-specific interactions between positively selected CD4(+) thymocytes bearing autoreactive T cell receptor (TCR) and mTECs displaying cognate self-peptide-MHC class II complexes. These interactions also involve the engagement of CD40 on mTECs by CD40L induced on the positively selected CD4(+) thymocytes. This antigen-specific TCR-MHC class II-mediated crosstalk between CD4(+) thymocytes and mTECs defines a unique checkpoint in thymic stromal development that is pivotal for generating a mature mTEC population competent for ensuring central T cell tolerance.
Publisher
CELL PRESS
Keywords
CLASS-II TRANSACTIVATOR; PROMISCUOUS GENE-EXPRESSION; T-CELLS; SELF-TOLERANCE; IN-VIVO; POSITIVE SELECTION; DENDRITIC CELLS; DEFICIENT MICE; PROMOTER-IV; MHC
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Creation Date: 2008-09-19 12:00:00
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