Blood-stage Plasmodium infection induces CD8(+) T lymphocytes to parasite-expressed antigens, largely regulated by CD8 alpha(+) dendritic cells

Lundie, RJ; de Koning-Ward, TF; Davey, GM; Nie, CQ; Hansen, DS; Lau, LS; Mintern, JD; Belz, GT; Schofield, L; Carbone, FR; Villadangos, JA; Crabb, BS; Heath, WR

Author(s): Lundie, RJ; de Koning-Ward, TF; Davey, GM; Nie, CQ; Hansen, DS; Lau, LS; Mintern, JD; Belz, GT; Schofield, L; Carbone, FR; Villadangos, JA; Crabb, BS; Heath, WR;
Details: Publication Year 2008-09-23,Volume 105,Issue #38,Page 14509-14514
Journal Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type: Journal Article
Abstract: Although CD8(+) T cells do not contribute to protection against the blood stage of Plasmodium infection, there is mounting evidence that they are principal mediators of murine experimental cerebral malaria (ECM). At present, there is no direct evidence that the CD8(+) T cells mediating ECM are parasite-specific or, for that matter, whether parasite-specific CD8(+) T cells are generated in response to blood-stage infection. To resolve this and to define the cellular requirements for such priming, we generated transgenic A berghei parasites expressing model T cell epitopes. This approach was necessary as MHC class I-restricted antigens to blood-stage infection have not been defined. Here, we show that blood-stage infection leads to parasite-specific CD8(+) and CD4(+) T cell responses. Furthermore, we show that P. berghei-expressed antigens are cross-presented by the CD8 alpha(+) subset of dendritic cells (DC), and that this induces pathogen-specific cytotoxic T lymphocytes (CTL) capable of lysing cells presenting antigens expressed by blood-stage parasites. Finally, using three different experimental approaches, we provide evidence that CTL specific for parasite-expressed antigens contribute to ECM.
Publisher: NATL ACAD SCIENCES
Keywords: EXPERIMENTAL CEREBRAL MALARIA; TUMOR-NECROSIS-FACTOR; CROSS-PRESENTATION; CUTTING EDGE; IN-VIVO; PATHOGENESIS; BERGHEI; SUSCEPTIBILITY; TRANSFECTION; INDUCTION
Publisher's Version: https://doi.org/10.1073/pnas.0806727105
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2008-09-23 12:00:00