Hls5 regulated erythroid differentiation by modulating GATA-1 activity
- Author(s)
- Endersby, R; Majewski, IJ; Winteringham, L; Beaumont, JG; Samuels, A; Scaife, R; Lim, E; Crossley, M; Klinken, SP; Lalonde, JP;
- Details
- Publication Year 2008-02-15,Volume 111,Issue #4,Page 1946-1950
- Journal Title
- BLOOD
- Publication Type
- Journal Article
- Abstract
- Hemopoietic lineage switch (His) 5 and 7 were originally isolated as genes upregulated during an erythroid-to-myeloid lineage switch. We have shown previously that HIs7/Mlf1 imposes a monoblastoid phenotype on erythroleukemic cells. Here we show that Hls5 impedes erythroid maturation by restricting proliferation and inhibiting hemoglobin synthesis; however, HIs5 does not influence the morphology of erythroid cells. Under the influence of GATA-1, HIs5 relocates from cytoplasmic granules to the nucleus where it associates with both FOG-1 and GATA-1. In the nucleus, HIs5 is able to suppress GATA-1-mediated transactivation and reduce GATA-1 binding to DNA. We conclude that HIs5 and Hls7/Mlf1 act cooperatively to induce biochemical and phenotypic changes associated with erythroid/myeloid lineage switching.
- Publisher
- AMER SOC HEMATOLOGY
- Keywords
- HEMATOPOIETIC LINEAGE SWITCH; TRANSCRIPTION FACTOR GATA-1; FINGER PROTEIN; RING FINGER; B-BOX; CELLS; ERYTHROPOIETIN; GENE; PROLIFERATION; ACTIVATION
- Publisher's Version
- https://doi.org/10.1182/blood-2007-04-085746
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2008-02-15 12:00:00