Multiple Dendritic Cell Populations Activate CD4(+) T Cells after Viral Stimulation
- Author(s)
- Mount, AM; Smith, CM; Kupresanin, F; Stoermer, K; Heath, WR; Belz, GT;
- Details
- Publication Year 2008-02-27,Volume 3,Issue #2,Page -
- Journal Title
- PLOS ONE
- Publication Type
- Journal Article
- Abstract
- Dendritic cells (DC) are a heterogeneous cell population that bridge the innate and adaptive immune systems. CD8a DC play a prominent, and sometimes exclusive, role in driving amplification of CD8(+) T cells during a viral infection. Whether this reliance on a single subset of DC also applies for CD4(+) T cell activation is unknown. We used a direct ex vivo antigen presentation assay to probe the capacity of flow cytometrically purified DC populations to drive amplification of CD4(+) and CD8(+) T cells following infection with influenza virus by different routes. This study examined the contributions of non-CD8 alpha DC populations in the amplification of CD8(+) and CD4(+) T cells in cutaneous and systemic influenza viral infections. We confirmed that in vivo, effective immune responses for CD8(+) T cells are dominated by presentation of antigen by CD8a DC but can involve non-CD8a DC. In contrast, CD4(+) T cell responses relied more heavily on the contributions of dermal DC migrating from peripheral lymphoid tissues following cutaneous infection, and CD4 DC in the spleen after systemic infection. CD4(+) T cell priming by DC subsets that is dependent upon the route of administration raises the possibility that vaccination approaches could be tailored to prime helper T cell immunity.
- Publisher
- PUBLIC LIBRARY SCIENCE
- Publisher's Version
- https://doi.org/10.1371/journal.pone.0001691
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2008-02-27 12:00:00