Spatial dissection of the cis- and trans-Golgi compartments in the malaria parasite Plasmodium falciparum
- Struck, NS; Herrmann, S; Schmuck-Barkmann, I; Dias, SD; Haase, S; Cabrera, AL; Treeck, M; Bruns, C; Langer, C; Cowman, AF; Marti, M; Spielmann, T; Gilberger, TW;
Publication Year 2008-03, Volume 67, Issue #6, Page 1320-1330
- Journal Title
- MOLECULAR MICROBIOLOGY
- Publication Type
- Journal Article
- The Golgi apparatus forms the heart of the secretory pathway in eukaryotic cells where proteins are modified, processed and sorted. The transport of proteins from the endoplasmic reticulum (ER) to the cis-side of the Golgi complex takes place at specialized ER sub-domains known as transitional ER (tER). We used the Plasmodium falciparum orthologue of Sec13p to analyse tER organization. We show that the distribution of PfSec13p is restricted to defined areas of the ER membrane. These foci are juxtaposed to the Golgi apparatus and might represent tER sites. To further analyse cis- to trans-Golgi architecture, we generated a double transfectant parasite line that expresses the Golgi marker Golgi reassembly stacking protein (GRASP) as a green fluorescent protein fusion and the trans-Golgi marker Rab6 as a DsRed fusion protein. Our data demonstrate that Golgi multiplication is closely linked to tER multiplication, and that parasite maturation is accompanied by the spatial separation of the cis- and trans- face of this organelle.
- BLACKWELL PUBLISHING
- TOXOPLASMA-GONDII; PICHIA-PASTORIS; ENDOPLASMIC-RETICULUM; SECRETORY PATHWAY; RAB GTPASES; INFECTED ERYTHROCYTES; TRYPANOSOMA-CRUZI; PROTEINS; LOCALIZATION; COMPLEX
- Publisher's Version
- Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2008-03-01 12:00:00Last Modified: 0001-01-01 12:00:00