Truncation of Plasmodium berghei merozoite surface protein 8 does not affect in vivo blood-stage development
Details
Publication Year 2008-05,Volume 159,Issue #1,Page 69-72
Journal Title
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Publication Type
Journal Article
Abstract
Merozoite surface protein 8 (MSP8) has shown promise as a vaccine candidate in the Plasmodium yoelii rodent malaria model and has a proposed role in merozoite invasion of erythrocytes. However, the temporal expression and localisation of MSP8 are unusual for a merozoite antigen. Moreover, in Plasmodium falciparum the MSP8 gene could be disrupted with no apparent effect on in vitro growth. To address the in vivo function of full-length MSP8, we truncated MSP8 in the rodent parasite Plasmodium berghei. Pb Delta MSP8 disruptant parasites displayed a normal blood-stage growth rate but no increase in reticulocyte preference, a phenomenon observed in P. yoelii MSP8 vaccinated mice. Expression levels of erythrocyte surface antigens were similar in P. berghei wild-type and Pb Delta MSP8-infected erythrocytes, suggesting that a parasitophorous vacuole function for MSP8 does not involve global trafficking of such antigens. These data demonstrate that a full-length membrane-associated form of PbMSP8 is not essential for blood-stage growth. (C) 2008 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Keywords
MEROZOITE SURFACE PROTEIN-8; DETERGENT-RESISTANT MEMBRANES; FACTOR-LIKE DOMAINS; INFECTED ERYTHROCYTES; INHIBITORY ANTIBODIES; MALARIA PARASITES; AOTUS-NANCYMAI; FALCIPARUM; INVASION; PROTECTION
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Creation Date: 2008-05-01 12:00:00
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