Fatty acids from Plasmodium falciparum down-regulate the toxic activity of malaria glycosylphosphatidylinositols
- Author(s)
- Debierre-Grockiego, F; Schofield, L; Azzouz, N; Schmidt, J;
- Details
- Publication Year 2006-10,Volume 74,Issue #10,Page 5487-5496
- Journal Title
- INFECTION AND IMMUNITY
- Publication Type
- Journal Article
- Abstract
- Plasmodium falciparum malaria kills roughly 2.5 million people, mainly children, annually. Much of this mortality is thought to arise from the actions of a malarial toxin. This toxin, identified as glycosylphosphatidylinositol (GPI), is a major pathogenicity determinant in malaria. A malarial molecule, Pfj, labeled by [H-3]glucosamine like the GPIs, was identified as a non-GPI molecule. Here we show that Pfj is able to down-regulate tumor necrosis factor alpha (TNF-alpha) production induced by the GPI of P. falciparum. Mass spectrometry analysis showed that Pfj was not a single molecule but represented a number of molecules. Separation methods, such as cation-exchange chromatography and thin-layer chromatography, were used to isolate and identify the following four main fatty acids responsible for the inhibitory effect on TNF-alpha production: myristic, pentadecanoic, palmitic, and palmitoleic acids. This regulatory effect on cytokine production suggests that there is balanced bioactivity for the different categories of malarial lipids.
- Publisher
- AMER SOC MICROBIOLOGY
- Keywords
- ASEXUAL ERYTHROCYTIC STAGES; VASCULAR ENDOTHELIAL-CELLS; CONJUGATED LINOLEIC-ACID; FACTOR-ALPHA PRODUCTION; PROTEIN-KINASE-C; INFECTED ERYTHROCYTES; PARASITIZED ERYTHROCYTES; SIGNAL-TRANSDUCTION; MEMBRANE ANCHOR; PPAR-ALPHA
- Publisher's Version
- https://doi.org/10.1128/IAI.01934-05
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2006-10-01 12:00:00