Normalization of boutique two-color microarrays with a high proportion of differentially expressed probes
- Author(s)
- Oshlack, A; Emslie, D; Corcoran, LM; Smyth, GK;
- Details
- Publication Year 2007,Volume 8,Issue #1,Page -
- Journal Title
- GENOME BIOLOGY
- Publication Type
- Journal Article
- Abstract
- Normalization is critical for removing systematic variation from microarray data. For two-color microarray platforms, intensity-dependent lowess normalization is commonly used to correct relative gene expression values for biases. Here we outline a normalization method for use when the assumptions of lowess normalization fail. Specifically, this can occur when specialized boutique arrays are constructed that contain a subset of genes selected to test particular biological functions.
- Publisher
- BIOMED CENTRAL LTD
- Keywords
- CDNA MICROARRAY; HOUSEKEEPING GENES
- Publisher's Version
- https://doi.org/10.1186/gb-2007-8-1-r2
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2007-01-01 12:00:00