Development of recombinant protein-based influenza vaccine Expression and affinity purification of H1N1 influenza virus neuraminidase

Dalakouras, T; Smith, BJ; Platis, D; Cox, MMJ; Labrou, NE

Author(s): Dalakouras, T; Smith, BJ; Platis, D; Cox, MMJ; Labrou, NE;
Details: Publication Year 2006-12-08,Volume 1136,Issue #1,Page 48-56
Journal Title: JOURNAL OF CHROMATOGRAPHY A
Publication Type: Journal Article
Abstract: The influenza virus surface glycoprotein antigen neuraminidase (NA) is a crucial viral enzyme with many potential medical applications; therefore, the development of efficient upstream and downstream processing strategy for the expression and purification of NA is of high importance. In the present work the NA gene from the H1N1 influenza vir-us strain A/Beijing/262/95 was cloned from viral RNA and expressed in expresSF+ insect cells using the baculovirus expression vector system (BVES). A limited affinity-ligand library was synthesized and evaluated for its ability to bind and purify the recombinant H1N1 neuraminidase. Affinity-ligand design was based on mimicking the interactions of the lock-and-key (LAK) motif (Phe-Gly-Gln), a common structural moiety found in the subunit interface of glutathione S-transferase I (GST 1), and plays an important structural role in subunit-subunit recognition. Solid-phase combinatorial chemistry was used to synthesize 13 variants of the lock-and-key lead ligand (Phe-Trz-X, where X was selected alpha-amino acid) using the 1,3,5-triazine moiety (Trz) as the scaffold for assembly. One immobilized ligand, bearing phenylalanine and isoleucine linked on the chlorotriazine ring (Phe-Trz-Ile), displayed high affinity for NA. Absorption equilibrium and molecular modeling studies were carried out to provide a detailed picture of Phe-Trz-Ile interaction with NA. This LAK-mimetic affinity adsorbent was exploited in the development of a facile purification protocol for NA, which led to 335-fold purification in a single-step. The present purification procedure is the most efficient reported so far for recombinant NA. (c) 2006 Elsevier B.V. All rights reserved.
Publisher: ELSEVIER SCIENCE BV
Keywords: A VIRUS; LIGAND INTERACTIONS; AUTOMATED DOCKING; SUBUNIT INTERFACE; SCORING FUNCTION; KEY MOTIF; CHROMATOGRAPHY; BINDING; ADSORBENTS; DESIGN
Publisher's Version: https://doi.org/10.1016/j.chroma.2006.09.067
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2006-12-08 12:00:00