The dominant role of CD8(+) dendritic cells in cross-presentation is not dictated by antigen capture
- Author(s)
- Schnorrer, P; Behrens, GMN; Wilson, NS; Pooley, JL; Smith, CM; EI-Sukkari, D; Davey, G; Kupresanin, F; Li, M; Maraskovsky, E; Belz, GT; Carbone, FR; Shortman, K; Heath, WR; Villadangos, JA;
- Details
- Publication Year 2006-07-11,Volume 103,Issue #28,Page 10729-10734
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- Mouse spleens contain three populations of conventional (CD11c(high)) dendritic cells (DCs) that play distinct functions. The CID8(+) DC are unique in that they can present exogenous antigens on their MIHC class I molecules, a process known as cross-presentation. It is unclear whether this special ability is because only the CD8(+) DC can capture the antigens used in cross-presentation assays, or because this is the only DC population that possesses specialized machinery for cross-presentation. To solve this important question we examined the splenic DC subsets for their ability to both present via MHC class II molecules and cross-present via MHC class I using four different forms of the model antigen ovalbumin (OVA). These forms include a cell-associated form, a soluble form, OVA expressed in bacteria, or OVA bound to latex beads. With the exception of bacterial antigen, which was poorly cross-presented by all DC, all antigenic forms were cross-presented much more efficiently by the CD8+ DC. This pattern could not be attributed simply to a difference in antigen capture because all DC subsets presented the antigen via MIHC class II. Indeed, direct assessments of endocytosis showed that CD8(+) and CD8(-) DC captured comparable amounts of soluble and bead-associated antigen, yet only the CD8+ DC cross-presented these antigenic forms. Our results indicate that cross-presentation requires specialized machinery that is expressed by CD8+ DC but largely absent from CD8(-) DC. This conclusion has important implications for the design of vaccination strategies based on antigen targeting to DC.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- MHC CLASS-I; T-CELLS; EXOGENOUS ANTIGENS; IMMUNE-COMPLEXES; TUMOR-ANTIGEN; CUTTING EDGE; NOD MICE; VIVO; MOUSE; MOLECULES
- Publisher's Version
- https://doi.org/10.1073/pnas.0601956103
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2006-07-11 12:00:00