Intrasplenic steady-state dendritic cell precursors that are distinct from monocytes
- Author(s)
- Naik, SH; Metcalf, D; van Nieuwenhuijze, A; Wicks, I; Wu, L; O'Keeffe, M; Shortman, K;
- Details
- Publication Year 2006-06,Volume 7,Issue #6,Page 663-671
- Journal Title
- NATURE IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Immediate precursors of the many subtypes of dendritic cells (DCs) remain obscure. Here we purified a splenic precursor population that produced all splenic CD8(+) and CD8(-) conventional DCs (cDCs) but not plasmacytoid DCs or other lineages. This 'pre-cDC' population included cells 'precommitted' to form either CD8(+) or CD8(-) cDCs. The pre-cDCs, which comprised 0.05% of splenocytes, expressed a CD11c(int)CD45RA(Io)CD43(int)SIRP-alpha(int)CD4(-)CD8(-) major histocompatibility complex class II-negative surface phenotype. The pre-cDCs were not monocytes. Monocytes generated few cDCs in steady-state recipient mice. However, when transferred into mice with an inflammatory milieu dependent on granulocyte-macrophage colony-stimulating factor, monocytes produced a distinct type of splenic DC. Thus, the inflammatory status of the host influences the developmental origin and type of DC present in lymphoid tissues.
- Publisher
- NATURE PUBLISHING GROUP
- Keywords
- COLONY-STIMULATING FACTOR; MOUSE LYMPHOID ORGANS; BONE-MARROW; IN-VIVO; HEMATOPOIETIC PRECURSORS; NITRIC-OXIDE; FLT3 LIGAND; T-CELLS; SPLEEN; DIFFERENTIATION
- Publisher's Version
- https://doi.org/10.1038/ni1340
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2006-06-01 12:00:00