Steroid hormone receptor status of mouse mammary stem cells

Asselin-Labat, ML; Shackleton, M; Stingl, J; Vaillant, F; Forrest, NC; Eaves, CJ; Visvader, JE; Lindeman, GJ

Author(s): Asselin-Labat, ML; Shackleton, M; Stingl, J; Vaillant, F; Forrest, NC; Eaves, CJ; Visvader, JE; Lindeman, GJ;
Details: Publication Year 2006-07-19,Volume 98,Issue #14,Page 1011-1014
Journal Title: JOURNAL OF THE NATIONAL CANCER INSTITUTE
Publication Type: Journal Article
Abstract: The estrogen receptor alpha (ER alpha), progesterone receptor (PR), and erbB2 (Her2 in humans) are important prognostic markers of human breast cancer, and they are variably expressed in different subtypes of breast cancer. The basal subtype, for example, is negative for ERa, PR, and Her2 by immunohistochemistry. We investigated the expression of these signaling molecules in enriched populations of mouse mammary stem cells and luminal cells that were isolated according to their differential expression of CD24 and the alpha 6 beta 1-integrin complex. We found that the basal population, which is enriched in mouse mammary stem cells, did not express ERa, PR, or ErbB2/Her2 but did express epidermal growth factor receptor (EGFR)/ ErbB1, whereas the subset of cells enriched for luminal cells expressed ERa (37% of cells) and PR (40% of cells) but not ErbB2/Her2 or EGFR/ErbB1. Ovariectomy confirmed the importance of estrogen signaling to luminal cell proliferation but had no effect on the size of the mouse mammary stem cell-enriched population. Thus, mouse mammary stem cells were negative for ER alpha, PR, and ErbB2 and appeared to share common properties with poor-prognosis basal breast cancer.
Publisher: OXFORD UNIV PRESS INC
Keywords: BREAST-CANCER; MOLECULAR MARKERS; EXPRESSION; GLAND; BRCA1; PROTOONCOGENE; OOPHORECTOMY; CARCINOMAS; MUTATIONS; PHENOTYPE
Publisher's Version: https://doi.org/10.1093/jnci/djj267
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2006-07-19 12:00:00