Characterisation of duplicate zinc finger like 2 erythroid precursor genes in zebrafish

Author(s): Hogan, BM; Pase, L; Hall, NE; Lieschke, GJ;
Details: Publication Year 2006-09,Volume 216,Issue #9,Page 523-529
Journal Title: DEVELOPMENT GENES AND EVOLUTION
Publication Type: Journal Article
Abstract: In separate expression pattern and micro-array screens the zinc finger containing factor, znfl2, has been previously implicated in hematopoiesis. Here we analysed znfl2 expression in detail and performed genetic epistatic analysis in a series of hematopoietic mutants and transient gain-of-function models. znfl2 expression in the hematopoietic intermediate mesoderm and derived erythrocytes required early genes cloche and spadetail, but not gata1. Expression was up-regulated in scl gain-of-function embryos, identifying znfl2 as an early erythroid factor that is regulated upstream or independently of gata1. Furthermore, we identified a duplicate znfl2 gene in the genome (znfl2b) which was expressed in early mesendoderm and weakly in the lateral plate mesoderm, overlapping in expression with znfl2. The production of loss-of-function models for znfl2, znfl2b and znfl2/znfl2b together suggested that these erythrocyte specific zinc finger genes are dispensible for erythropoiesis.
Publisher: SPRINGER
Keywords: CLOCHE; ERYTHROPOIESIS; HEMATOPOIESIS; DIFFERENTIATION; PROGENITORS; MESODERM; GATA1
Publisher's Version: https://doi.org/10.1007/s00427-006-0062-y
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2006-09-01 12:00:00