Evidence that Plasmodium falciparum chromosome end clusters are cross-linked by protein and are the sites of both virulence gene silencing and activation
Details
Publication Year 2006-10, Volume 62, Issue #1, Page 72-83
Journal Title
MOLECULAR MICROBIOLOGY
Publication Type
Journal Article
Abstract
The malaria parasite Plasmodium falciparum undergoes antigenic variation through allelic exclusion and variant expression of surface proteins encoded by the var gene family. Regulation of var genes is under epigenetic control and involves reversible silencing and activation that requires the physical repositioning of a var locus into a transcriptionally permissive zone of the nuclear periphery. P. falciparum chromosome ends appear to aggregate into large perinuclear clusters which house both subtelomeric and chromosome central var genes. In this study we further define the composition of telomeric clusters using fluorescent in situ hybridization, and provide evidence that chromosome end clusters are formed by cross-linking protein. In addition, we demonstrate that a subtelomeric reporter gene and a var gene remain within clusters regardless of their transcriptional status. Our findings support a model whereby a highly localized structure dedicated to the activation of a single var gene can be housed within a gene dense chromosome end cluster that is otherwise transcriptionally silent.
Publisher
BLACKWELL PUBLISHING
Keywords
CHONDROITIN-SULFATE-A; ERYTHROCYTE-MEMBRANE PROTEIN-1; ANTIGENIC VARIATION; INFECTED ERYTHROCYTES; YEAST TELOMERES; SUBTELOMERIC REGIONS; MALARIA PARASITES; NUCLEAR-ENVELOPE; SIR4 PROTEINS; EXPRESSION
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Creation Date: 2006-10-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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