The Ets factor Spi-B is a direct critical target of the coactivator OBF-1
- Author(s)
- Bartholdy, B; Du Roure, C; Bordon, A; Emslie, D; Corcoran, LM; Matthias, P;
- Details
- Publication Year 2006-08-01,Volume 103,Issue #31,Page 11665-11670
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- OBF-1 (Bob.1, OCA-B) is a lymphoid-specific transcriptional coactivator that associates with the transcription factors Oct-1 or Oct-2 on the conserved octamer element present in the promoters of several ubiquitous and lymphoid-specific genes. OBF-1-deficient mice have B cell-intrinsic defects, lack germinal centers, and have severely impaired immune responses to T cell-dependent antigens. Crucial genes that are regulated by OBF-1 and that might explain the observed phenotype of OBF-1 deficiency have remained elusive to date. Here we have generated transgenic mice expressing OBF-1 specifically in T cells and examined these together with mice lacking OBF-1 to discover transcriptional targets of this coactivator. Using microarray analysis, we have identified the Ets transcription factor Spi-B as a direct target gene critically regulated by OBF-1 that can help explain the phenotype of OBF-1-deficient mice. Spi-B has been implicated in signaling pathways downstream of the B cell receptor and is essential for germinal center formation and maintenance. The present findings establish a hierarchy between these two factors and provide a molecular link between OBF-1 and B cell receptor signaling.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- CELL-SPECIFIC COACTIVATOR; IMMUNOGLOBULIN GENE-TRANSCRIPTION; OCA-B; PROMOTER ACTIVITY; MICE LACKING; POU DOMAIN; T-CELLS; EXPRESSION; BOB.1/OBF.1; RECEPTOR
- Publisher's Version
- https://doi.org/10.1073/pnas.0509430103
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2006-08-01 12:00:00