Rapid specific amplification of rat antibody cDNA from nine hybridomas in the presence of myeloma light chains

Author(s): Brady, JL; Corbett, AJ; McKenzie, BS; Lew, AM;
Details: Publication Year 2006-08-31,Volume 315,Issue #1-2,Page 61-67
Journal Title: JOURNAL OF IMMUNOLOGICAL METHODS
Publication Type: Journal Article
Abstract: Most monoclonal antibodies to mouse antigens have been derived from rat spleen-mouse myeloma fusions. Many resultant hybridomas express one of several myeloma kappa chain transcripts, even though the parent myeloma may have been ascribed as not expressing light chain protein. Previous reports have only differentiated against one of these mouse light chains. We have found at least three different myeloma kappa transcripts in the panel of nine hybridomas that were derived from four different myeloma parents. We have designed an amplification strategy that differentiates the rearranged rat kappa chain from all mouse light chains. Moreover, this method is expedient as it requires minimal downstream manipulation. (c) 2006 Published by Elsevier B.V.
Publisher: ELSEVIER SCIENCE BV
Keywords: VARIABLE REGIONS; PCR AMPLIFICATION; MOLECULAR-CLONING; RELIABLE CLONING; KAPPA-CHAIN; CELL-LINES; SEQUENCE; DIGESTION; COMPLEX
Publisher's Version: https://doi.org/10.1016/j.jim.2006.07.002
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2006-08-31 12:00:00