RANK signals from CD4(+)3(-) inducer cells regulate development of Aire-expressing epithelial cells in the thymic medulla
Details
Publication Year 2007-06-11,Volume 204,Issue #6,Page 1267-1272
Journal Title
JOURNAL OF EXPERIMENTAL MEDICINE
Publication Type
Journal Article
Abstract
Aire-expressing medullary thymic epithelial cells (mTECs) play a key role in preventing autoimmunity by expressing tissue-restricted antigens to help purge the emerging T cell receptor repertoire of self-reactive specificities. Here we demonstrate a novel role for a CD4(+)3(-) inducer cell population, previously linked to development of organized secondary lymphoid structures and maintenance of T cell memory in the functional regulation of Aire-mediated promiscuous gene expression in the thymus. CD4(+)3(-) cells are closely associated with mTECs in adult thymus, and in fetal thymus their appearance is temporally linked with the appearance of Aire(+) mTECs. We show that RANKL signals from this cell promote the maturation of RANK-expressing CD80(-) Aire(-) mTEC progenitors into CD80(+) Aire(+) mTECs, and that transplantation of RANK-deficient thymic stroma into immunodeficient hosts induces autoimmunity. Collectively, our data reveal cellular and molecular mechanisms leading to the generation of Aire+ mTECs and highlight a previously unrecognized role for CD4(+)3(-) RANKL(+) inducer cells in intrathymic self-tolerance.
Publisher
ROCKEFELLER UNIV PRESS
Keywords
PROMISCUOUS GENE-EXPRESSION; CD4(+)CD3(-) CELLS; AUTOIMMUNITY; STROMA; MATURATION; TOLERANCE; LIGAND; OX40
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Creation Date: 2007-06-11 12:00:00
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