CREB activity modulates neural cell proliferation, midbrain-hindbrain organization and patterning in zebrafish
Details
Publication Year 2007-07-01, Volume 307, Issue #1, Page 127-141
Journal Title
DEVELOPMENTAL BIOLOGY
Publication Type
Journal Article
Abstract
Neural stem/progenitor cells (NPCs) self-renew and differentiate, generating neuronal and non-neuronal (glial) cell lineages. Although a number of factors, including transcription factors, have been shown to be important in the regulation of NPC proliferation and differentiation, the precise molecular networks remain to be identified. The cAMP Response Element-Binding protein (CREB) is a transcription factor important for neuronal survival, differentiation and plasticity. Recent work suggests that CREB activation, via serine phosphorylation in the kinase inducible domain, is important for neurogenesis in the adult rodent brain. We sought to further investigate CREB function in neurogenesis, using the zebrafish (Danio rerio). Structural and functional analysis of the zebrafish CREB orthologue showed high conservation with mammalian CREB. Activated (phosphorylated) CREB (pCREB) was localised to all known proliferation zones in the adult zebrafish brain, including actively cycling cells. Furthermore, we found that modulating CREB activity during early zebrafish development caused significant defects in neural proliferation, midbrain-hindbrain organization and body patterning. These findings reveal broader and stage-specific physiological roles of CREB function during vertebrate neural development and proliferation. Crown Copyright (c) 2007 Published by Elsevier Inc. All rights reserved.
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Keywords
ELEMENT-BINDING PROTEIN; RESPONSE ELEMENT; STEM-CELLS; OLFACTORY-BULB; ADULT-RAT; SUBVENTRICULAR ZONE; NEWBORN NEURONS; NERVOUS-SYSTEM; SONIC-HEDGEHOG; DANIO-RERIO
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Creation Date: 2007-07-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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