Multiplex ligation-dependent probe amplification (MLPA) genotyping assay for mouse models of down syndrome
Journal Title
FRONTIERS IN BIOSCIENCE
Publication Type
Journal Article
Abstract
The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assigning genotypes. We report a 100% accurate multiplex ligation-dependent probe amplification (MLPA) assay for genotyping the Ts65Dn mouse that is also applicable to all other segmentally trisomic mouse models of Down syndrome.
Publisher
FRONTIERS IN BIOSCIENCE INC
Keywords
BEHAVIORAL ABNORMALITIES; TS65DN; IDENTIFICATION; PHENOTYPES; TS1CJE
Publisher's Version
https://doi.org/10.2741/2291
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2007-05-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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