The BH3-only protein bid is dispensable for DNA darnage- and replicative stress-induced apoptosis or cell-cycle arrest
Details
Publication Year 2007-04-20, Volume 129, Issue #2, Page 423-433
Journal Title
CELL
Publication Type
Journal Article
Abstract
Bid, a caspase-activated proapoptotic BH3 only protein, is essential for Fas-induced hepatocyte destruction. Recent studies published in Cell produced conflicting results, indicating that loss of Bid either protects or enhances apoptosis induced by DNA damage or replicative stress. To resolve this controversy, we generated novel Bid-deficient mice on an inbred C57BL/6 background and removed the drugselection cassette from the targeted locus. Nine distinct cell types from these Bid-deficient mice underwent cell-cycle arrest and apoptosis in a manner indistinguishable from control WT cells in response to DNA damage or replicative stress. Moreover, we found that even cells from the original Bid-deficient mice responded normally to these stimuli, indicating that differences in genetic background or the presence of a strong promoter within the targeted locus are unlikely to explain the differences between our results and those reported previously. We conclude that Bid has no role in DNA damage- or replicative stress-induced apoptosis or cellcycle arrest.
Publisher
CELL PRESS
Keywords
FAMILY-MEMBER BIM; DAMAGE RESPONSE; BH3 DOMAINS; BCL-2; DEATH; MITOCHONDRIA; DELETION; MOUSE; PUMA; HOMEOSTASIS
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2007-04-20 12:00:00
Last Modified: 0001-01-01 12:00:00
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