Different kinetics of Blimp-1 induction in B cell subsets revealed by reporter gene
- Author(s)
- Fairfax, KA; Corcoran, LM; Pridans, C; Huntington, ND; Kallies, A; Nutt, SL; Tarlinton, DM;
- Details
- Publication Year 2007-04-01,Volume 178,Issue #7,Page 4104-4111
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- The transcriptional repressor Blimp-1 (B lymphocyte-induced maturation protein 1) has been described as a "master regulator" of B cell differentiation into Ab-secreting cells (ASCs). Although there is mounting evidence for the importance and necessity of Blimp-1 in plasma cell development, there is uncertainty as to the role it plays in B cell differentiation of B cell subsets and the way in which it may interact with other transcription factors such as Pax5 and Bcl6 during ASC differentiation. Using a mouse expressing GFP under the control of the Blimp-1 regulatory elements (Blimp-1(GFP/+)), we examined the kinetics of Blimp-1 up-regulation in purified B cell subsets following activation. B1 cells showed the most rapid and pronounced up-regulation of Blimp-1 in response to the mitogens tested, followed by marginal zone B cells and then conventional B2 cells. Interestingly, only Ill cells substantially up-regulated Blimp-1 expression in response to CpG. B1 cells secreted negligible Ig upon isolation but were able to up-regulate Blimp-1 and initiate Ig secretion within 28 h of stimulation. Also of interest, B1 cells have a transcriptional factor profile that is intermediate between a naive B cell and an ASC, indicative of the semiactivated state of B1 cells. Transferred naive Blimp-1(GFP/+) B1 and B2 cells both gave rise to ASCs in the bone marrow, suggesting no intrinsic barriers to BI cell entry into the long-lived ASC compartment.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- MARGINAL ZONE; PLASMA-CELLS; LYMPHOCYTE DIFFERENTIATION; B-1 CELLS; SECRETORY APPARATUS; MICE; EXPRESSION; ACTIVATION; MECHANISMS; PHENOTYPE
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Creation Date: 2007-04-01 12:00:00