Essential role of the PfRh5/PfRipr/CyRPA complex during Plasmodium falciparum invasion of erythrocytes
Details
Publication Year 2016-06-30, Volume 20, Issue #1, Page 60-71
Journal Title
Cell Host Microbe
Publication Type
Journal Article
Abstract
Plasmodium falciparum parasites in the merozoite stage invade human erythrocytes and cause malaria. Invasion requires multiple interactions between merozoite ligands and erythrocyte receptors. P. falciparum reticulocyte binding homolog 5 (PfRh5) forms a complex with the PfRh5-interacting protein (PfRipr) and Cysteine-rich protective antigen (CyRPA) and binds erythrocytes via the host receptor basigin. However, the specific role that PfRipr and CyRPA play during invasion is unclear. Using P. falciparum lines conditionally expressing PfRipr and CyRPA, we show that loss of PfRipr or CyRPA function blocks growth due to the inability of merozoites to invade erythrocytes. Super-resolution microscopy revealed that PfRipr, CyRPA, and PfRh5 colocalize at the junction between merozoites and erythrocytes during invasion. PfRipr, CyRPA, and PfRipr/CyRPA/PfRh5-basigin complex is required for triggering the Ca2+ release and establishing the tight junction. Together, these results establish that the PfRh5/PfRipr/CyRPA complex is essential in the sequential molecular events leading to parasite invasion of human erythrocytes.
Publisher
Cell Press
WEHI Research Division(s)
Systems Biology And Personalised Medicine; Infection And Immunity
PubMed ID
27374406
NHMRC Grants
NHMRC/637406 NHMRC/1026581
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2016-08-09 12:01:43
Last Modified: 2016-08-10 09:46:17
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