Synthesis of a tethered myo-Inositol (1,3,4,5,6)Pentakisphosphate (IP5) derivative as a probe for biological studies
- Author(s)
- Gregory, M; Catimel, B; Yin, MX; Condron, M; Burgess, AW; Holmes, AB;
- Details
- Publication Year 2016,Volume 27,Issue #01,Page 121-125
- Journal Title
- Synlett
- Publication Type
- Journal Article
- Abstract
- There is sufficient evidence to suggest that myo-inositol pentakisphosphate is a vital intermediate species in higher inositol phosphate metabolism, however, its biological roles and physiological function in cells remain uncertain. A tethered myo-inositol pentakisphosphate (IP5) derivative with a terminal amine group is synthesised allowing facilitated immobilisation onto M-270 magnetic Dynabeads for pull-down experiments and biosensor chip preparation for surface plasmon resonance studies. The probes are validated by both pull-down and surface plasmon resonance (SPR) studies of the known binding protein GRP-1 (general receptor for phosphoinositides 1), and furthermore by SPR studies of protein kinase B (PKB or AKT) binding.
- Publisher
- Thieme
- Research Division(s)
- Structural Biology
- Publisher's Version
- https://doi.org/10.1055/s-0035-1560381
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2016-06-15 08:51:08
Last Modified: 2016-06-15 09:02:41