BAX-BAK1-independent LC3B lipidation by BH3 mimetics is unrelated to BH3 mimetic activity and has only minimal effects on autophagic flux
- Author(s)
- Reljic, B; Conos, S; Lee, EF; GARNIER, JM; Dong, L; Lessene, G; Fairlie, WD; Vaux, DL; Lindqvist, LM;
- Journal Title
- Autophagy
- Publication Type
- Journal Article
- Abstract
- Inhibition of prosurvival BCL2 family members can induce autophagy, but the mechanism is controversial. We have provided genetic evidence that BCL2 family members block autophagy by inhibiting BAX and BAK1, but others have proposed they instead inhibit BECN1. Here we confirm that small molecule BH3 mimetics can induce BAX- and BAK1-independent MAP1LC3B/LC3B lipidation, but this only occurred at concentrations far greater than required to induce apoptosis and dissociate canonical BH3 domain-containing proteins that bind more tightly than BECN1. Because high concentrations of a less-active enantiomer of ABT-263 also induced BAX- and BAK1-independent LC3B lipidation, induction of this marker of autophagy appears to be an off-target effect. Indeed, robust autophagic flux was not induced by BH3 mimetic compounds in the absence of BAX and BAK1. Therefore at concentrations that are on target and achievable in vivo, BH3 mimetics only induce autophagy in a BAX- and BAK1-dependent manner.
- Publisher
- Taylor & Francis
- Research Division(s)
- Cell Signalling And Cell Death; Structural Biology; Chemical Biology
- PubMed ID
- 27172402
- Link To PubMed Central Version
- https://www-ncbi-nlm-nih-gov/pmc/articles/PMC4990995/
- Publisher's Version
- https://doi.org/10.1080/15548627.2016.1179406
- NHMRC Grants
- NHMRC/1020136, NHMRC/1049949, NHMRC/1035502, NHMRC/1024620,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2016-06-15 12:27:25
Last Modified: 2018-01-19 09:00:13