Higher-resolution structure of the human insulin receptor ectodomain: multi-modal inclusion of the insert domain
- Author(s)
- Croll, TI; Smith, BJ; Margetts, MB; Whittaker, J; Weiss, MA; Ward, CW; Lawrence, MC;
- Details
- Publication Year 2016-03-01,Volume 24,Issue #3,Page 469-76
- Journal Title
- Structure
- Publication Type
- Journal Article
- Abstract
- Insulin receptor (IR) signaling is critical to controlling nutrient uptake and metabolism. However, only a low-resolution (3.8 A) structure currently exists for the IR ectodomain, with some segments ill-defined or unmodeled due to disorder. Here, we revise this structure using new diffraction data to 3.3 A resolution that allow improved modeling of the N-linked glycans, the first and third fibronectin type III domains, and the insert domain. A novel haptic interactive molecular dynamics strategy was used to aid fitting to low-resolution electron density maps. The resulting model provides a foundation for investigation of structural transitions in IR upon ligand binding.
- Publisher
- Cell Press
- Research Division(s)
- Structural Biology
- PubMed ID
- 26853939
- Link To PubMed Central Version
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860004/
- Publisher's Version
- https://doi.org/10.1016/j.str.2015.12.014
- NHMRC Grants
- NHMRC/1005896, NHMRC/1058233,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2016-03-14 03:05:29
Last Modified: 2018-03-08 11:36:00