Higher-resolution structure of the human insulin receptor ectodomain: multi-modal inclusion of the insert domain
Details
Publication Year 2016-03-01, Volume 24, Issue #3, Page 469-76
Journal Title
Structure
Publication Type
Journal Article
Abstract
Insulin receptor (IR) signaling is critical to controlling nutrient uptake and metabolism. However, only a low-resolution (3.8 A) structure currently exists for the IR ectodomain, with some segments ill-defined or unmodeled due to disorder. Here, we revise this structure using new diffraction data to 3.3 A resolution that allow improved modeling of the N-linked glycans, the first and third fibronectin type III domains, and the insert domain. A novel haptic interactive molecular dynamics strategy was used to aid fitting to low-resolution electron density maps. The resulting model provides a foundation for investigation of structural transitions in IR upon ligand binding.
Publisher
Cell Press
WEHI Research Division(s)
Structural Biology
PubMed ID
26853939
NHMRC Grants
NHMRC/1005896 NHMRC/1058233
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2016-03-14 03:05:29
Last Modified: 2018-03-08 11:36:00
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