Different regions of Plasmodium falciparum Erythrocyte binding antigen-175 induce antibody responses to infection of varied efficacy
Journal Title
J Infect Dis
Publication Type
Journal Article in press
Abstract
BACKGROUND: Increasing evidence suggests that antibodies against merozoite proteins involved inP. falciparuminvasion into the red blood cell (RBC) play an important role in clinical immunity to malaria. Erythrocyte binding antigen 175 (EBA-175) is the best-characterizedP. falciparuminvasion ligand, described to recognize glycophorin A on the surface of erythrocytes. The protein structure of EBA-175 comprises six extracellular regions. While region II (RII) contains Duffy Binding Like domains involved in the binding to glycophorin A, the functional role of regions III-V is less clear. METHODS: Here we developed a novel cytometric bead array for assessment of antigen-specific antibody concentration in plasma to evaluate the efficacy of immune responses to different regions of EBA-175 and associations between antibody levels with protection from symptomatic malaria in a treatment-reinfection cohort study. RESULTS: We found that while antibodies to the RII are highly abundant, circulating levels as low as 5-10microg/ml of antibodies specific for region III (RIII) or the highly conserved region IV-V (RIV-V) predict strong protection from clinical malaria. CONCLUSIONS: These results lend support for the development of conserved regions of EBA-175 as components in a combination of a malaria vaccine.
Publisher
OUP
WEHI Research Division(s)
Infection And Immunity; Population Health And Immunity
PubMed ID
27020092
NHMRC Grants
NHMRC/1043345 NHMRC/1058665
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2016-04-05 01:43:59
Last Modified: 2016-04-05 02:43:03
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