Using CRISPR/Cas9 technology for manipulating cell death regulators
- Author(s)
- Kueh, AJ; Herold, MJ;
- Journal Title
- Methods in Molecular Biology
- Publication Type
- Journal Article
- Abstract
- Clustered, regularly interspaced, short palindromic repeats (CRISPR)/Cas9 technology has been demonstrated to be a useful tool for generating targeted mutations in cell lines and mice. However, the use of CRISPR/Cas9 in a constitutively expressed manner can often result in low targeting efficiencies and lethality due to mutations in essential genes. Here, we describe the use of an inducible lentiviral vector platform, enabling rapid transduction and enrichment of CRISPR/Cas9 positive cells and high levels of targeted mutations upon induction.
- Publisher
- Springer
- Research Division(s)
- Molecular Genetics Of Cancer
- PubMed ID
- 27108444
- Publisher's Version
- https://doi.org/10.1007/978-1-4939-3581-9_18
- NHMRC Grants
- NHMRC/1016701, NHMRC/1049720,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2016-05-02 10:21:20
Last Modified: 2018-11-30 01:56:26