Proteomic analysis reveals novel proteins associated with the Plasmodium protein exporter PTEX and a loss of complex stability upon truncation of the core PTEX component, PTEX150
- Author(s)
- Elsworth, B; Sanders, PR; Nebl, T; Batinovic, S; Kalanon, M; Nie, CQ; Charnaud, SC; Bullen, HE; de Koning Ward, TF; Tilley, L; Crabb, BS; Gilson, PR;
- Details
- Publication Year 2016-03-28,Volume 18,Issue #11,Page 1551-1569
- Journal Title
- Cellular Microbiology
- Publication Type
- Journal Article
- Abstract
- The Plasmodium translocon for exported proteins (PTEX) has been established as the machinery responsible for the translocation of all classes of exported proteins beyond the parasitophorous vacuolar membrane of the intraerythrocytic malaria parasite. Protein export, particularly in the asexual blood stage, is crucial for parasite survival as exported proteins are involved in remodelling the host cell, an essential process for nutrient uptake, waste removal and immune evasion. Here we have truncated the conserved C-terminus of one of the essential PTEX components, PTEX150, in Plasmodium falciparum in an attempt to create mutants of reduced functionality. Parasites tolerated C-terminal truncations of up to 125 amino acids with no reduction in growth, protein export or the establishment of new permeability pathways. Quantitative proteomic approaches however revealed a decrease in other PTEX subunits associating with PTEX150 in truncation mutants, suggesting a role for the C-terminus of PTEX150 in regulating PTEX stability. Our analyses also reveal three previously unreported PTEX-associated proteins, namely PV1, Pf113 and Hsp70-x (respective PlasmoDB numbers; PF3D7_1129100, PF3D7_1420700 and PF3D7_0831700), and demonstrate that core PTEX proteins exist in various distinct multimeric forms outside the major complex.
- Publisher
- Wiley
- Research Division(s)
- Systems Biology And Personalised Medicine
- PubMed ID
- 27019089
- Publisher's Version
- https://doi.org/10.1111/cmi.12596
- NHMRC Grants
- NHMRC/1068287, NHMRC/1021560, NHMRC/637406,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2016-04-05 01:43:56
Last Modified: 2018-07-06 09:58:25