Lineage tracing of mammary stem and progenitor cells
Journal Title
Methods Mol Biol
Publication Type
Journal Article
Abstract
Lineage tracing analysis allows mammary epithelial cells to be tracked in their natural environment, thereby revealing cell fate and proliferation choices in the intact tissue. This technique is particularly informative for studying how stem cells build and maintain the mammary epithelium during development and pregnancy. Here we describe two experimental systems based on Cre/loxP technology (CreERT2/loxP and rtTA/tetO-Cre/loxP), which allow the inducible, permanent labeling of mammary epithelial cells following the administration of either tamoxifen or doxycycline.
Publisher
Springer
Research Division(s)
Stem Cells And Cancer
PubMed ID
27796960
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2017-05-26 03:11:45
Last Modified: 2017-05-26 03:47:24
An error has occurred. This application may no longer respond until reloaded. Reload 🗙