Laser-mediated rupture of chlamydial inclusions triggers pathogen egress and host cell necrosis
Journal Title
Nat Commun
Publication Type
Journal Article
Abstract
Remarkably little is known about how intracellular pathogens exit the host cell in order to infect new hosts. Pathogenic chlamydiae egress by first rupturing their replicative niche (the inclusion) before rapidly lysing the host cell. Here we apply a laser ablation strategy to specifically disrupt the chlamydial inclusion, thereby uncoupling inclusion rupture from the subsequent cell lysis and allowing us to dissect the molecular events involved in each step. Pharmacological inhibition of host cell calpains inhibits inclusion rupture, but not subsequent cell lysis. Further, we demonstrate that inclusion rupture triggers a rapid necrotic cell death pathway independent of BAK, BAX, RIP1 and caspases. Both processes work sequentially to efficiently liberate the pathogen from the host cytoplasm, promoting secondary infection. These results reconcile the pathogen's known capacity to promote host cell survival and induce cell death.
Publisher
Springer Nature
Research Division(s)
Cell Signalling And Cell Death
PubMed ID
28281536
Open Access at Publisher's Site
https://www-nature-com/articles/ncomms14729
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2017-04-12 10:42:09
Last Modified: 2017-04-12 11:00:14
An error has occurred. This application may no longer respond until reloaded. Reload 🗙