Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells
- Author(s)
- Choi, YJ; Lin, CP; Risso, D; Chen, S; Kim, TA; Tan, MH; Li, JB; Wu, Y; Chen, C; Xuan, Z; Macfarlan, T; Peng, W; Lloyd, KC; Kim, SY; Speed, TP; He, L;
- Details
- Publication Year 2017-02-10,Volume 355,Issue #6325,Page doi: 10.1126/science.aag1927
- Journal Title
- Science
- Publication Type
- Journal Article
- Abstract
- Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) efficiently generate all embryonic cell lineages but rarely generate extraembryonic cell types. We found that microRNA miR-34a deficiency expands the developmental potential of mouse pluripotent stem cells, yielding both embryonic and extraembryonic lineages and strongly inducing MuERV-L (MERVL) endogenous retroviruses, similar to what is seen with features of totipotent two-cell blastomeres. miR-34a restricts the acquisition of expanded cell fate potential in pluripotent stem cells, and it represses MERVL expression through transcriptional regulation, at least in part by targeting the transcription factor Gata2. Our studies reveal a complex molecular network that defines and restricts pluripotent developmental potential in cultured ESCs and iPSCs.
- Publisher
- AAAS
- Research Division(s)
- Bioinformatics
- PubMed ID
- 28082412
- Publisher's Version
- https://doi.org/10.1126/science.aag1927
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2017-04-06 09:28:02
Last Modified: 2017-04-07 03:27:09