Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive
Details
Publication Year 2017-03, Volume 19, Issue #3, Page 164-176
Journal Title
Nature Cell Biology
Publication Type
Journal Article
Abstract
Despite accumulating evidence for a mammary differentiation hierarchy, the basal compartment comprising stem cells remains poorly characterized. Through gene expression profiling of Lgr5+ basal epithelial cells, we identify a new marker, Tetraspanin8 (Tspan8). Fractionation based on Tspan8 and Lgr5 expression uncovered three distinct mammary stem cell (MaSC) subsets in the adult mammary gland. These exist in a largely quiescent state but differ in their reconstituting ability, spatial localization, and their molecular and epigenetic signatures. Interestingly, the deeply quiescent MaSC subset (Lgr5+Tspan8hi) resides within the proximal region throughout life, and has a transcriptome strikingly similar to that of claudin-low tumours. Lgr5+Tspan8hi cells appear to originate from the embryonic mammary primordia before switching to a quiescent state postnatally but can be activated by ovarian hormones. Our findings reveal an unexpected degree of complexity within the adult MaSC compartment and identify a dormant subset poised for activation in response to physiological stimuli.
Publisher
Springer Nature
WEHI Research Division(s)
Stem Cells And Cancer; Bioinformatics; Molecular Medicine
PubMed ID
28192422
Publisher's Version
https://doi.org/10.1038/ncb3471
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2017-04-06 09:27:48
Last Modified: 2018-03-20 01:27:19
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