Human RORgammat(+)CD34(+) cells are lineage-specified progenitors of group 3 RORgammat(+) innate lymphoid cells
Details
Publication Year 2014-12-18,Volume 41,Issue #6,Page 988-1000
Journal Title
Immunity
Publication Type
Journal Article
Abstract
Group 3 innate lymphoid cells (ILC3s) are defined by the expression of the transcription factor RORgammat, which is selectively required for their development. The lineage-specified progenitors of ILC3s and their site of development after birth remain undefined. Here we identified a population of human CD34(+) hematopoietic progenitor cells (HPCs) that express RORgammat and share a distinct transcriptional signature with ILC3s. RORgammat(+)CD34(+) HPCs were located in tonsils and intestinal lamina propria (LP) and selectively differentiated toward ILC3s. In contrast, RORgammat(-)CD34(+) HPCs could differentiate to become either ILC3s or natural killer (NK) cells, with differentiation toward ILC3 lineage determined by stem cell factor (SCF) and aryl hydrocarbon receptor (AhR) signaling. Thus, we demonstrate that in humans RORgammat(+)CD34(+) cells are lineage-specified progenitors of IL-22(+) ILC3s and propose that tonsils and intestinal LP, which are enriched both in committed precursors and mature ILC3s, might represent preferential sites of ILC3 lineage differentiation.
Publisher
Cell Press
Research Division(s)
Molecular Immunology
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2015-03-12 03:59:01
Last Modified: 2015-03-16 09:14:52
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