Investigating bak/bax activating conformation change by immunoprecipitation
- Author(s)
- Dewson, G;
- Details
- Publication Year 2015-05-01,Volume 2015,Issue #5,Page 472-476
- Journal Title
- Cold Spring Harb Protoc
- Publication Type
- Journal Article
- Abstract
- Activation of both Bax and Bak during apoptosis involves significant conformation change. Investigation of this phenomenon by immunoprecipitation (IP) requires a detergent such as CHAPS that does not induce significant conformation change. IP with conformation-specific Bax or Bak antibodies is observed in CHAPS only following an apoptotic stimulus, whereas the same antibodies will immunoprecipitate from both nonapoptotic and apoptotic cells in the presence of Triton X-100. Thus, the latter detergent can serve as a positive control for IP, as described here.
- Publisher
- CSHLP
- Research Division(s)
- Cell Signalling And Cell Death
- PubMed ID
- 25934937
- Publisher's Version
- https://doi.org/10.1101/pdb.prot086454
- NHMRC Grants
- NHMRC/637335,
- ARC Grants
- ARC/FT100100791,
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2015-05-22 11:19:35
Last Modified: 2016-01-21 08:49:10